Lineweaver–Burk Plot Because Vmax is attained at endless substrate concentration, estimating Vmax and thus Km from a hyperbolic plot is impossible. Due to this issue, Lineweaver and Burk modified the Michaelis–Menten equation into a straight line equation. The Lineweaver–Burk plot (also known as the double reciprocal plot) is a pictorial depiction of the Lineweaver–Burk equation … Read more
Flagella and Cilia Definition Cilia and Flagella are cytoplasmic filamentous structures which protrude through the cell wall. They are the cell’s minute, highly distinct appendages. An entire cell is propelled by its flagella (singular form: flagellum), which are long, hair-like projections that protrude from the plasma membrane. Small hairlike structures called cilia (singular = cilium) … Read more
Definition of p –value Calculated probabilities such as p-values can be utilised to detect the least possible level of significance for rejecting the null hypothesis. Under ideal circumstances, the test results will be identical to what was observed, assuming the null hypothesis is true. An extremely low p-value suggests that the outcome is conceivable, but … Read more
Nucleosome Model of Chromosome Chrosomes are self-replicating nucleic acid molecules that have particular organisational and functional characteristics and are essential for inheritance, mutation, variety and evolutionary advancement. Every chromosome’s structure is supported by many layers of DNA wrapped around proteins. Histones and non-histone chromosomal proteins are the traditional classifications for proteins which attach to DNA … Read more
What Is Northern Blot? For the study of RNA in complicated mixtures, Northern blot is a technique that relies on the notion of “blotting”. Essentially, it is a modified version of the Southern Blotting technique, which was originally developed for the examination of DNA sequences. Molecular biology requires the detection of certain sequences of nucleic … Read more
Mendel’s Laws of Segregation, Independent Assortment and Dominance This new theory of inheritance was first proposed in the early 1860s by an Austrian monk named Gregor Mendel depending on his experiments with pea plants at the University of Vienna. Heredity was Mendel’s belief, and each unit (or gene) in an individual’s genome acted independently of … Read more
Definition of Bacterial Transformation Bacterial transformation is the movement of free DNA from donor bacteria into the extracellular environment, resulting in absorption and, in most cases, the expression of the newly acquired characteristic in a recipient bacterium. There is no requirement for a live donor cell for this procedure, and just free DNA in the … Read more
Definition of Simple Squamous Epithelium Simple squamous epithelium, a kind of simple epithelium, is made by a single layer of cells on a basement membrane. It is a form of epithelium made up of a single layer of squamous or flat cells on top of a thin extracellular layer called the basement membrane. Such epithelium … Read more
Definition of Homeostasis Homeostasis refers to the capability of living systems to keep a constant as well as homogeneous internal environment in order for the systems to function normally. It is the desire to find balance in the face of various environmental as well as natural influences. Homeostasis produces a dynamic equilibrium wherein continual changes … Read more
Definition of Specification Speciation is the process of evolution which results in the emergence of a new genetically independent group of creatures known as a species. Speciation is the process of dividing a genetically homogeneous population into two or more populations, each of which undergoes genetic differentiation as well as eventually reproductive isolation. The creation … Read more